tRNA pseudouridine32 synthase
Identifiers
EC no.5.4.99.28
CAS no.430429-15-5
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
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PMCarticles
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NCBIproteins

tRNA pseudouridine32 synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine32 uracil mutase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

tRNA uridine32 tRNA pseudouridine32

The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine746 in 23S rRNA.

References

  1. Hoang C, Chen J, Vizthum CA, Kandel JM, Hamilton CS, Mueller EG, Ferré-D'Amaré AR (November 2006). "Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure". Molecular Cell. 24 (4): 535–45. doi:10.1016/j.molcel.2006.09.017. PMID 17188032.
  2. Spedaliere CJ, Hamilton CS, Mueller EG (August 2000). "Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues". Biochemistry. 39 (31): 9459–65. doi:10.1021/bi001079n. PMID 10924141.
  3. Raychaudhuri S, Niu L, Conrad J, Lane BG, Ofengand J (July 1999). "Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA". The Journal of Biological Chemistry. 274 (27): 18880–6. doi:10.1074/jbc.274.27.18880. PMID 10383384.
  4. Ramamurthy V, Swann SL, Spedaliere CJ, Mueller EG (October 1999). "Role of cysteine residues in pseudouridine synthases of different families". Biochemistry. 38 (40): 13106–11. doi:10.1021/bi9913911. PMID 10529181.
  5. Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J (June 1995). "A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe)". RNA. 1 (4): 437–48. PMC 1482406. PMID 7493321.
  6. Behm-Ansmant I, Grosjean H, Massenet S, Motorin Y, Branlant C (December 2004). "Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae" (PDF). The Journal of Biological Chemistry. 279 (51): 52998–3006. doi:10.1074/jbc.m409581200. PMID 15466869. S2CID 13197875.
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