Peptide therapeutics are peptides or polypeptides (oligomers or short polymers of amino acids) which are used to for the treatment of diseases. Naturally occurring peptides may serve as hormones, growth factors, neurotransmitters, ion channel ligands, and anti-infectives; peptide therapeutics mimic such functions. Peptide Therapeutics are seen as relatively safe and well-tolerated as peptides can be metabolized by the body.[1]

Examples

The current highest selling marketed diabetic drug Liraglutide, incorporates a lipid chain to extent plasma circulation and ensures prolonged bioavailability.[2][3] Liraglutide is a GLP-1 agonist drug that self-assembles into an alpha-helical structure, and it requires once a day administration.[4] Lipid conjugation of a palmitoyl chain to a lysine residue at position 26 of Liraglutide results in an extended half-life (around 13–14 hours) in the blood. This is due to the palmitoyl chain allowing non covalent binding to albumin, which delays proteolytic attack by DPP IV and also rapid renal clearance. Furthermore, the addition of the lipid chain could further prolong half-life by sterically hindering the DPP IV enzyme from degradation.[5]

Another peptide known to self-assemble is the octapeptide Lanreotide. This compound is a synthetic analogue of the peptide hormone somatostatin and it is used to treat acromegaly[6] (a condition where the body produced too much growth hormone). In water, Lanreotide self-assembles into monodisperse liquid crystalline nanotubes. The nanotubes are made up of dimers that self-assemble into a 2D crystal, which is held together by lateral chain interactions, and also by antiparallel ß-sheets.[6][7]

Further insight into how self-assembly and peptide hormones are related has been provided by studies on self-assembling amyloid structures formed by peptide hormones and neuropeptides. Peptide hormones and neuropeptides form dense-cored aggregates that pack into dense-core vesicles (DCVs), which are used to temporarily store peptide messengers in secretory cells.[8] When dense-core vesicles are triggered, they release the stored information into the blood or extracellular space,[9] resulting in amyloid disassembly, in order for action.[8] Therefore, for these types of peptides, reversibility of peptide aggregation is essential for their function.

Increasing stability of peptide drugs

Many strategies have been employed to increase the stability of peptide drugs, because although they have so many desirable characteristics, they are short lived in the body as a result of rapid degradation and clearance. With half-lives of some peptides and proteins only being a few minutes, they are very ineffective in drug delivery.[10] Mechanisms involved in their clearance include peripheral blood mediated elimination by proteolysis, real and hepatic elimination, and also receptor-mediated endocytosis.[11] One of the main reasons for such rapid clearance is molecular weight. Molecules that have a low molecular weight (40-50 kDa) are rapidly cleared by renal filtration via the glomerular filtration barrier (GBM) into the urine. As a result of this, increasing the size of a peptide drug is a good starting point to improve half-life.[12]

Peptide modifications to extend half-life include PEGylation, glycosylation, cyclization, serum albumin binding, and lipidation. PEGylation is the attachment of polyethylene glycol (PEG) chains to the peptide via covalent bonds, helping to increase molecular weight, and limit enzymatic degradation as a result of steric hindrance caused by adding the PEG.[13] PEGylation offers a number of benefits for pharmaceutical applications such as improved water solubility, high mobility in solution, as well as low toxicity and low immunogenicity. This does however depend on the molecular weight of the attached PEG.[14][15] PEGylation as a method to improve half-life has been successfully demonstrated many times; in one example it was shown that site specific mono-PEGylation of GLP-1 led to a 16-fold increase in plasma half life time in rats.[16] On the other hand, covalently attaching PEG can often lead to loss of biological activity.[17]

Another chemical modification is the attachment of glycosyl (carbohydrate) units to the peptide to help with peptide delivery to target sites. The introduction of carbohydrates to peptides can alter the physiological properties, to improve bioavailability. Advantages of this technique include increased metabolic stability, and facilitated transport across cell membranes, although of the most favourable aspects is their ability to promote oral absorption.[18] Peptides have a very low oral availability (less than 1-2%),[19][20][21] as a result of insufficient absorption and rapid degradation and clearance, thus making this method an attractive one. N- and O-glycosylation in which carbohydrates are attached to the peptide are naturally occurring, where N-glycosylation occurs through the amine group of an asparagine residue to form an amide bond. O-glycosylation occurs via serine or threonine residues, where the oxygen atom on the side chain binds to the carbohydrate through an ether bond. There is also non-natural glycosylation, known as chemical glycosylation, which involves the attachment of carbohydrate units to different amino acid residues at the N-terminus of the peptide's sequence. A further way of carrying out glycosylation is by using enzymes, known as chemo-enzymatic glycosylation. This method is used for complex chemical synthesis.[22][23] Chemical and chemo-enzymatic methods can be used for the synthesis of glycopeptides and glycoproteins.[18]

Cyclization can also be used as a method to decrease proteolytic degradation and prolong half-life, to make the peptide conformation more rigid to hinder enzymatic cleavage. This method can however lead to loss of biological function due to the reduced flexibility making the peptide inactive.[24] For example, side chain to side chain cyclization between asparagine (position 8) and lysine (position 12), of a growth regulating factor (GRF) analogue was found to increase the half-life from 17 minutes to more than 2 hours.[14]

Another way to extend half-life do is to bind serum albumin to the peptide. Human serum albumin is the most abundant plasma protein with a molecular weight of 66.4 kDa,[25] and it is involved in many essential bodily functions to maintain homeostasis. As a result, albumin binding would significantly increase the molecular weight of the peptide, restricting it from being filtered into the urine by the GBM. Serum albumin has an extraordinary long half-life of 2-4 weeks which is much longer than other plasma proteins,[26] due to it binding to the neonatal Fc receptor (FcRn). Fc receptors are proteins found on the surface of certain cells that help to protect the functions of the immune system, by binding to the Fc region of antibodies, which attach to pathogens and destroy them. This mechanism of the neonatal FcRn involves albumin binding to the FcRn in an acidic pH environment to divert it from degradation in the lysosomal compartment of the cell, and redirecting it to the plasma membrane, where it is released back into the blood plasma due to neutral pH.[27]

Lipidation is a further technique to use when improving peptide stability and half-life. Attaching a lipid chain to the peptide head group has been found to inhibit proteolytic attack due to the lipid chain non-covalently interacting with serum albumin to increase the molecular weight, thus reducing renal filtration. Studies on a lipidated analogue of insulin, detemir, revealed a prolonged action as a result of its affinity for human serum albumin.[28] As well as this, lipidation has been shown to enhance the interaction of peptides with cell membranes, allowing them to be up taken into the cell more readily compared to the peptide lacking the lipid moiety.[29][30] There are three types of lipidation, and they differ based on the bond formation methods between the lipid and the peptide: amidation, esterification (S- or O-) and S-bond (ether or disulphide) formation. Amidation and O-esterification form strong covalent bonds that are irreversible, whereas the other two methods are weak and reversible covalent bonds. The method used, as well as the alkyl/lipid chain, position of lipidation, and the spacer used, all have significant impacts on physiochemical properties and bioactivity.[31] The level of lipophilicity can be significantly modulated by lipidation, and since lipophilicity is detrimental for the absorption, distribution, metabolism, and excretion of drugs, it provides a way of fine tuning peptides for use in therapeutics .

A study on lipidation and PEGylation on the GLP-1 peptide was carried out and the results showed that lipidation had no significant effect on peptide activity in vitro,[32] whereas PEGylation did, especially when the PEG is attached to internal amino acids of the peptide e.g. positions 20 and 21. The reduction in activity from PEGylation compared to lipidation is due to the loss of receptor affinity, and it is suggested that this is because of its increased molecular weight which causes steric hindrance.[33][34]

References

 This article incorporates text by Jessica Hutchinson available under the CC BY-SA 3.0 license.

  1. "What are Peptide Therapeutics?". News-Medical.net. 2020-07-21. Retrieved 2021-03-06.
  2. Li Y, Shao M, Zheng X, Kong W, Zhang J, Gong M (September 2013). "Self-assembling peptides improve the stability of glucagon-like peptide-1 by forming a stable and sustained complex". Molecular Pharmaceutics. 10 (9): 3356–65. doi:10.1021/mp4001734. PMID 23859692.
  3. Gao Z, Bai G, Chen J, Zhang Q, Pan P, Bai F, Geng P (March 2009). "Development, characterization, and evaluation of a fusion protein of a novel glucagon-like peptide-1 (GLP-1) analog and human serum albumin in Pichia pastoris". Bioscience, Biotechnology, and Biochemistry. 73 (3): 688–94. doi:10.1271/bbb.80742. PMID 19270384. S2CID 39307659.
  4. Wang Y, Lomakin A, Kanai S, Alex R, Benedek GB (February 2015). "Transformation of oligomers of lipidated peptide induced by change in pH". Molecular Pharmaceutics. 12 (2): 411–9. doi:10.1021/mp500519s. PMID 25569709.
  5. Frederiksen TM, Sønderby P, Ryberg LA, Harris P, Bukrinski JT, Scharff-Poulsen AM, et al. (September 2015). "Oligomerization of a Glucagon-like Peptide 1 Analog: Bridging Experiment and Simulations". Biophysical Journal. 109 (6): 1202–13. Bibcode:2015BpJ...109.1202F. doi:10.1016/j.bpj.2015.07.051. PMC 4576320. PMID 26340816.
  6. 1 2 Valéry C, Artzner F, Robert B, Gulick T, Keller G, Grabielle-Madelmont C, et al. (April 2004). "Self-association process of a peptide in solution: from beta-sheet filaments to large embedded nanotubes". Biophysical Journal. 86 (4): 2484–501. Bibcode:2004BpJ....86.2484V. doi:10.1016/S0006-3495(04)74304-0. PMC 1304096. PMID 15041685.
  7. Gobeaux F, Fay N, Tarabout C, Meneau F, Mériadec C, Delvaux C, et al. (February 2013). "Experimental observation of double-walled peptide nanotubes and monodispersity modeling of the number of walls". Langmuir. 29 (8): 2739–45. doi:10.1021/la304862f. PMID 23368945.
  8. 1 2 Nespovitaya N, Gath J, Barylyuk K, Seuring C, Meier BH, Riek R (January 2016). "Dynamic Assembly and Disassembly of Functional β-Endorphin Amyloid Fibrils". Journal of the American Chemical Society. 138 (3): 846–56. doi:10.1021/jacs.5b08694. PMID 26699104.
  9. Glombik M (April 2000). "Signal-mediated sorting of neuropeptides and prohormones:Secretory granule biogenesis revisited". Biochimie. 82 (4): 315–326. doi:10.1016/S0300-9084(00)00195-4. PMID 10865120.
  10. Werle M, Bernkop-Schnürch A (June 2006). "Strategies to improve plasma half life time of peptide and protein drugs". Amino Acids. 30 (4): 351–67. doi:10.1007/s00726-005-0289-3. PMID 16622600. S2CID 31092931.
  11. Tang L, Persky AM, Hochhaus G, Meibohm B (September 2004). "Pharmacokinetic aspects of biotechnology products". Journal of Pharmaceutical Sciences. 93 (9): 2184–204. doi:10.1002/jps.20125. PMID 15295780.
  12. Tryggvason K, Wartiovaara J (April 2005). "How does the kidney filter plasma?". Physiology. 20 (2): 96–101. doi:10.1152/physiol.00045.2004. PMID 15772298.
  13. Hamley IW (May 2014). "PEG-peptide conjugates" (PDF). Biomacromolecules. 15 (5): 1543–59. doi:10.1021/bm500246w. PMID 24720400. S2CID 28954181.
  14. 1 2 Werle M, Bernkop-Schnürch A (June 2006). "Strategies to improve plasma half life time of peptide and protein drugs". Amino Acids. 30 (4): 351–67. doi:10.1007/s00726-005-0289-3. PMID 16622600. S2CID 31092931.
  15. Bellmann-Sickert K, Elling CE, Madsen AN, Little PB, Lundgren K, Gerlach LO, et al. (April 2011). "Long-acting lipidated analogue of human pancreatic polypeptide is slowly released into circulation". Journal of Medicinal Chemistry. 54 (8): 2658–67. doi:10.1021/jm101357e. PMID 21410292.
  16. Lee SH, Lee S, Youn YS, Na DH, Chae SY, Byun Y, Lee KC (2005-03-01). "Synthesis, characterization, and pharmacokinetic studies of PEGylated glucagon-like peptide-1". Bioconjugate Chemistry. 16 (2): 377–82. doi:10.1021/bc049735+. PMID 15769092.
  17. Harris JM, Chess RB (March 2003). "Effect of pegylation on pharmaceuticals". Nature Reviews. Drug Discovery. 2 (3): 214–21. doi:10.1038/nrd1033. PMID 12612647. S2CID 574824.
  18. 1 2 Moradi SV, Hussein WM, Varamini P, Simerska P, Toth I (April 2016). "Glycosylation, an effective synthetic strategy to improve the bioavailability of therapeutic peptides". Chemical Science. 7 (4): 2492–2500. doi:10.1039/C5SC04392A. PMC 5477030. PMID 28660018.
  19. Mandal D, Nasrolahi Shirazi A, Parang K (June 2014). "Self-assembly of peptides to nanostructures". Organic & Biomolecular Chemistry. 12 (22): 3544–61. doi:10.1039/C4OB00447G. PMC 4038164. PMID 24756480.
  20. Zhou P, Deng L, Wang Y, Lu JR, Xu H (February 2016). "Different nanostructures caused by competition of intra- and inter-β-sheet interactions in hierarchical self-assembly of short peptides". Journal of Colloid and Interface Science. 464: 219–28. Bibcode:2016JCIS..464..219Z. doi:10.1016/j.jcis.2015.11.030. PMID 26619132.
  21. Houston ME, Wallace A, Bianchi E, Pessi A, Hodges RS (September 1996). "Use of a conformationally restricted secondary structural element to display peptide libraries: a two-stranded alpha-helical coiled-coil stabilized by lactam bridges". Journal of Molecular Biology. 262 (2): 270–82. doi:10.1006/jmbi.1996.0512. PMID 8831793.
  22. Salamone S, Guerreiro C, Cambon E, André I, Remaud-Siméon M, Mulard LA (February 2015). "Programmed chemo-enzymatic synthesis of the oligosaccharide component of a carbohydrate-based antibacterial vaccine candidate". Chemical Communications. 51 (13): 2581–4. doi:10.1039/C4CC08805K. PMID 25569152.
  23. Fujikawa K, Koizumi A, Hachisu M, Seko A, Takeda Y, Ito Y (February 2015). "Construction of a high-mannose-type glycan library by a renewed top-down chemo-enzymatic approach". Chemistry. 21 (8): 3224–33. doi:10.1002/chem.201405781. PMID 25586968.
  24. Räder AF, Reichart F, Weinmüller M, Kessler H (June 2018). "Improving oral bioavailability of cyclic peptides by N-methylation". Bioorganic & Medicinal Chemistry. 26 (10): 2766–2773. doi:10.1016/j.bmc.2017.08.031. PMID 28886995. S2CID 29149595.
  25. Ehrlich GK, Michel H, Truitt T, Riboulet W, Pop-Damkov P, Goelzer P, et al. (December 2013). "Preparation and characterization of albumin conjugates of a truncated peptide YY analogue for half-life extension". Bioconjugate Chemistry. 24 (12): 2015–24. doi:10.1021/bc400340z. PMID 24251972.
  26. Kontermann RE (2009-04-01). "Strategies to extend plasma half-lives of recombinant antibodies". BioDrugs. 23 (2): 93–109. doi:10.2165/00063030-200923020-00003. PMID 19489651. S2CID 12722160.
  27. Kontermann RE (December 2011). "Strategies for extended serum half-life of protein therapeutics". Current Opinion in Biotechnology. 22/6 Chemical biotechnology and Pharmaceutical biotechnology. 22 (6): 868–76. doi:10.1016/j.copbio.2011.06.012. PMID 21862310.
  28. van Witteloostuijn SB, Pedersen SL, Jensen KJ (November 2016). "Half-Life Extension of Biopharmaceuticals using Chemical Methods: Alternatives to PEGylation". ChemMedChem. 11 (22): 2474–2495. doi:10.1002/cmdc.201600374. PMID 27775236. S2CID 205649390.
  29. Makovitzki A, Baram J, Shai Y (October 2008). "Antimicrobial lipopolypeptides composed of palmitoyl Di- and tricationic peptides: in vitro and in vivo activities, self-assembly to nanostructures, and a plausible mode of action". Biochemistry. 47 (40): 10630–6. doi:10.1021/bi8011675. PMID 18783248.
  30. Epand, Richard M. (1997). "Biophysical studies of lipopeptide-membrane interactions". Peptide Science. 43 (1): 15–24. doi:10.1002/(SICI)1097-0282(1997)43:1<15::AID-BIP3>3.0.CO;2-3. ISSN 1097-0282. PMID 9174409.
  31. Bulaj, L. Zhang and G. (2012-03-31). "Converting Peptides into Drug Leads by Lipidation". Current Medicinal Chemistry. 19 (11): 1602–1618. doi:10.2174/092986712799945003. PMID 22376031. Retrieved 2021-01-20.
  32. Knudsen LB, Nielsen PF, Huusfeldt PO, Johansen NL, Madsen K, Pedersen FZ, et al. (May 2000). "Potent derivatives of glucagon-like peptide-1 with pharmacokinetic properties suitable for once daily administration". Journal of Medicinal Chemistry. 43 (9): 1664–9. doi:10.1021/jm9909645. PMID 10794683.
  33. Pan CQ, Buxton JM, Yung SL, Tom I, Yang L, Chen H, et al. (May 2006). "Design of a long acting peptide functioning as both a glucagon-like peptide-1 receptor agonist and a glucagon receptor antagonist". The Journal of Biological Chemistry. 281 (18): 12506–15. doi:10.1074/jbc.M600127200. PMID 16505481.
  34. Thorens B (September 1992). "Expression cloning of the pancreatic beta cell receptor for the gluco-incretin hormone glucagon-like peptide 1". Proceedings of the National Academy of Sciences of the United States of America. 89 (18): 8641–5. Bibcode:1992PNAS...89.8641T. doi:10.1073/pnas.89.18.8641. PMC 49976. PMID 1326760.
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